Enright Anne-Marie, Collins Gavin, O'Flaherty Vincent
Microbial Ecology Laboratory, Department of Microbiology and Environmental Change Institute, National University of Ireland, Galway, Galway, Ireland.
Syst Appl Microbiol. 2007 Sep;30(6):471-82. doi: 10.1016/j.syapm.2007.03.005. Epub 2007 May 2.
Anaerobic sludge granules were obtained from laboratory-scale anaerobic bioreactors used to treat pharmaceutical-like (methanol-, acetone- and propanol-contaminated) wastewater under low-temperature conditions (15 degrees C). The microbial diversity and diversity changes of the sludge samples were ascertained by applying 16S rRNA gene cloning and terminal restriction fragment length polymorphism (TRFLP) analyses, respectively, and using sludge samples from the inoculum, throughout and at the conclusion of the bioreactor trial. Data from genetic fingerprinting correlated well with those from physiological activity assays of the reactor biomass. Specifically, for example, TRFLP profiles indicated the dominance of hydrogenotrophic methanogens within the archaeal community, thus supporting the findings of specific methanogenic activity measurements. TRFLP data supported the hypothesis that the deviation between the replicated reactors, in terms of treatment efficiency, was associated with succession within the microbial communities present, and indicated that community development was linked to both operating temperature and wastewater composition. Fluorescence in situ hybridization (FISH) was also applied, to quantitatively assess the abundance of selected microbial groups, and revealed the underestimation of the abundance Methanosarcina by gene cloning analysis and demonstrated the spatial arrangement of these organisms within the architecture of the low-temperature solvent-degrading anaerobic biofilms.
厌氧污泥颗粒取自实验室规模的厌氧生物反应器，该反应器用于在低温条件（15摄氏度）下处理类似制药的（受甲醇、丙酮和丙醇污染的）废水。分别通过应用16S rRNA基因克隆和末端限制性片段长度多态性（TRFLP）分析，并使用接种物、生物反应器试验全过程及试验结束时的污泥样本，确定了污泥样本的微生物多样性及其变化。遗传指纹数据与反应器生物质生理活性测定数据高度相关。具体而言，例如，TRFLP图谱表明氢营养型产甲烷菌在古菌群落中占主导地位，从而支持了特定产甲烷活性测量的结果。TRFLP数据支持了这样的假设，即复制反应器在处理效率方面的差异与存在的微生物群落演替有关，并表明群落发展与操作温度和废水成分都有关联。还应用了荧光原位杂交（FISH）来定量评估选定微生物群的丰度，揭示了基因克隆分析对甲烷八叠球菌丰度的低估，并展示了这些生物体在低温溶剂降解厌氧生物膜结构内的空间排列。
