Ansaruzzaman Mohammad, Bhuiyan Nurul A, Safa Ashrafus, Sultana Marzia, McUamule Arminda, Mondlane Catarina, Wang Xuan-Yi, Deen Jacqueline L, von Seidlein Lorenz, Clemens John D, Lucas Marcelino, Sack David A, Balakrish Nair Gopinath
Laboratory Sciences Division, Centre for Health and Population Research, International Centre for Diarrhoeal Disease Research, Dhaka 1000, Bangladesh.
Int J Med Microbiol. 2007 Oct;297(6):443-9. doi: 10.1016/j.ijmm.2007.01.009. Epub 2007 May 1.
Vibrio cholerae O1 strains that are hybrids between the classical and El Tor biotypes were isolated during two consecutive years (2004-2005) from diarrheal patients in Mozambique. Similar variants isolated in Bangladesh and recently isolated El Tor strains were analyzed for genetic diversity. Pulsed-field gel electrophoresis (PFGE) analysis using the restriction enzyme NotI, resulted in 18-21 bands showed five closely related PFGE patterns that were distributed similarly in both years (2004-2005) among the 80 strains tested in Mozambique. Overall based on the PFGE patterns the hybrids indicated an El Tor lineage. The restriction patterns of whole-chromosomal DNA grouped the hybrid strains from Mozambique into a separate cluster from Bangladeshi clinical and environmental hybrid strains. A high molecular weight band of 398kb that contain rstR allele of the classical type was detected from all hybrid strains, which was absent in all conventional classical and El Tor strains. This band could be designated as a marker for the hybrid strains. This study suggests that hybrid strains from Mozambique are closely related to each other, different from Bangladeshi hybrid strains that are diverse in nature and all hybrid strains differed markedly from conventional classical and El Tor strains.
在连续两年（2004 - 2005年）期间，从莫桑比克腹泻患者中分离出了霍乱弧菌O1型菌株，这些菌株是古典生物型和埃尔托生物型之间的杂交种。对在孟加拉国分离出的类似变体以及最近分离出的埃尔托菌株进行了遗传多样性分析。使用限制性内切酶NotI进行脉冲场凝胶电泳（PFGE）分析，产生了18 - 21条带，显示出五种密切相关的PFGE模式，在2004 - 2005年这两年中，在莫桑比克测试的80株菌株中分布相似。总体而言，基于PFGE模式，这些杂交种显示出埃尔托谱系。全染色体DNA的限制性模式将来自莫桑比克的杂交菌株与孟加拉国临床和环境杂交菌株归为不同的簇。在所有杂交菌株中检测到一条398kb的高分子量带，其包含古典类型的rstR等位基因，而所有传统的古典菌株和埃尔托菌株中均不存在该带。这条带可被指定为杂交菌株的标志物。这项研究表明，来自莫桑比克的杂交菌株彼此密切相关，不同于本质上具有多样性的孟加拉国杂交菌株，并且所有杂交菌株与传统的古典菌株和埃尔托菌株明显不同。
