Levisetti Matteo G, Suri Anish, Petzold Shirley J, Unanue Emil R
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
J Immunol. 2007 May 15;178(10):6051-7. doi: 10.4049/jimmunol.178.10.6051.
Several naturally occurring anti-insulin CD4 T cells were isolated from islet infiltrates of NOD mice. In accordance with the results of others, these T cells recognized the segment of the beta-chain from residues 9-23. Peptides encompassing the B:(9-23) sequence bound weakly to I-Ag7 in two main contiguous registers in which two residues at the carboxyl end, P20Gly and P21Glu, influenced binding and T cell reactivity. Naturally occurring insulin-reactive T cells exhibited differing reactivities with the carboxyl-terminal amino acids, although various single residue changes in either the flanks or the core segments affected T cell responses. The insulin peptides represent another example of a weak MHC-binding ligand that is highly immunogenic, giving rise to distinct populations of autoimmune T cells.
从非肥胖糖尿病（NOD）小鼠的胰岛浸润物中分离出几种天然存在的抗胰岛素CD4 T细胞。与其他人的结果一致，这些T细胞识别β链9-23位残基的片段。包含B：（9-23）序列的肽在两个主要连续区域中与I-Ag7弱结合，其中羧基末端的两个残基P20Gly和P21Glu影响结合和T细胞反应性。天然存在的胰岛素反应性T细胞对羧基末端氨基酸表现出不同的反应性，尽管侧翼或核心片段中的各种单残基变化会影响T细胞反应。胰岛素肽代表了另一个弱MHC结合配体的例子，该配体具有高度免疫原性，可产生不同群体的自身免疫性T细胞。
