Benítez-King G, Huerto-Delgadillo L, Antón-Tay F
Departamento de Neurofarmacología, Instituto Mexicano de Psiquiatría, México.
Brain Res. 1991 Aug 23;557(1-2):289-92. doi: 10.1016/0006-8993(91)90146-m.
The interaction between melatonin and calmodulin was explored. Calmodulin cell levels in MDCK and N1E-115 cells cultured with 10(-9) M melatonin were increased after 3 days but decreased after 6 days. Melatonin inhibited calmodulin-dependent phosphodiesterase and when either melatonin or [3H]melatonin was preincubated with calmodulin and separated by electrophoresis, comigration of calmodulin with the radioactivity as well as modification of the Ca2+ calmodulin shift were observed. The results point out that one of the mechanisms of action of melatonin is a calmodulin-melatonin interaction.
研究了褪黑素与钙调蛋白之间的相互作用。用10⁻⁹ M褪黑素培养的MDCK和N1E - 115细胞中的钙调蛋白细胞水平在3天后升高，但在6天后降低。褪黑素抑制钙调蛋白依赖性磷酸二酯酶，当褪黑素或[³H]褪黑素与钙调蛋白预孵育并通过电泳分离时，观察到钙调蛋白与放射性物质的共迁移以及Ca²⁺ - 钙调蛋白迁移的改变。结果指出，褪黑素作用机制之一是钙调蛋白 - 褪黑素相互作用。
