Wu Jun-Zhe, Wu Wen-Hua, He Li-Jiang, Ke Qing-Feng, Huang Long, Dai Zhang-Sheng, Chen Yu
Department of Orthopaedics, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian 362000, China.
Department of Orthopaedics, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
Biomed Res Int. 2016;2016:8460291. doi: 10.1155/2016/8460291. Epub 2016 Nov 30.
. To explore influence of continuous illumination, luzindole, and Tamoxifen on incidence of scoliosis model of rats. . Thirty-two one-month-old female rats were rendered into bipedal rats. The bipedal rats were divided into 4 groups: group A by intraperitoneal injection of luzindole and continuous illumination; group B by intraperitoneal injection of luzindole only; group C by intraperitoneal injection of luzindole and oral administration of Tamoxifen; and group D by intraperitoneal injection of equivalent saline. Radiographs were taken at 8th week and 16th week, and incidence and the Cobb angles of scoliosis were calculated. At 16th week, all rats were sacrificed. Before the sacrifice, the levels of calmodulin were measured in each group. . At 8th week, scoliosis occurred in groups A and B, with an incidence of 75% and 12.5%, respectively, while rats in group C or D had no scoliosis. At 16th week, scoliosis incidences in groups A and B were 57% and 62.5%, respectively. No scoliosis occurred in group C or D. Calmodulin in platelets in group B was significantly different, compared with groups A and D. There was no significant difference in calmodulin in platelets in groups B and C. . By intraperitoneal injection of luzindole in bipedal rats, scoliosis rat models could be successfully made. Under light, incidence of scoliosis may be increased at an early period but it is reversible. Tamoxifen can suppress natural process of scoliosis.
探讨持续光照、鲁辛朵及他莫昔芬对大鼠脊柱侧弯模型发病率的影响。将32只1月龄雌性大鼠制成双足大鼠。双足大鼠分为4组：A组腹腔注射鲁辛朵并持续光照；B组仅腹腔注射鲁辛朵；C组腹腔注射鲁辛朵并口服他莫昔芬；D组腹腔注射等量生理盐水。于第8周和第16周拍摄X线片，计算脊柱侧弯的发病率及Cobb角。第16周时，处死所有大鼠。处死前，测量每组大鼠的钙调蛋白水平。第8周时，A组和B组出现脊柱侧弯，发病率分别为75%和12.5%，而C组或D组大鼠未出现脊柱侧弯。第16周时，A组和B组脊柱侧弯发病率分别为57%和62.5%。C组和D组未出现脊柱侧弯。B组血小板中的钙调蛋白与A组和D组相比有显著差异。B组和C组血小板中的钙调蛋白无显著差异。通过对双足大鼠腹腔注射鲁辛朵，可成功制作脊柱侧弯大鼠模型。在光照下，脊柱侧弯发病率在早期可能会增加，但具有可逆性。他莫昔芬可抑制脊柱侧弯的自然进程。
