Danks A M, Oestreicher A B, Spruijt B M, Gispen W H, Isaacson R L
State University of New York, Binghamton 13902-6000.
Brain Res. 1991 Aug 23;557(1-2):308-12. doi: 10.1016/0006-8993(91)90150-t.
Male Wistar rats subjected to unilateral fimbria-fornix transection by mechanical knife cut or to sham operations were tested in a water maze and in an open field. Half the animals in each group were treated with either 0.06 mg/kg nimodipine or vehicle, administered i.p. for 7 days, beginning the day of surgery. Animals were sacrificed and brains were processed for acetylcholine esterase (AChE) histochemistry. In the water maze, lesioned rats showed a significant impairment relative to the sham-operated animals. Nimodipine treatment did not improve performance. There were no differences among the groups in the observed frequencies of the open field behaviors of locomotion, hole-poke, rearing and grooming. A significant reduction of AChE-positive cell bodies was found in the medial septal region on the side of the lesion. There were no differences in water maze performance among groups of rats treated with 0.0, 0.5, 1.0, or 5.0 mg/kg nimodipine for 7 days, beginning the day of fimbria-fornix transection, in an attempt to determine any dose-dependent effect of the drug.
通过机械手术刀切断单侧穹窿海马伞或进行假手术的雄性Wistar大鼠，在水迷宫和旷场中接受测试。每组动物中有一半用0.06 mg/kg尼莫地平或赋形剂腹腔注射治疗7天，从手术当天开始。处死动物并对大脑进行乙酰胆碱酯酶（AChE）组织化学处理。在水迷宫中，与假手术动物相比，损伤大鼠表现出明显的损伤。尼莫地平治疗并未改善其表现。在旷场中观察到的运动、探洞、直立和梳理行为的频率在各组之间没有差异。在损伤侧的内侧隔区发现AChE阳性细胞体显著减少。为了确定该药物的任何剂量依赖性效应，从穹窿海马伞切断当天开始，对用0.0、0.5、1.0或5.0 mg/kg尼莫地平治疗7天的大鼠组进行水迷宫测试，结果各组之间在水迷宫表现上没有差异。
