Tsen Kong T, Dykeman Eric C, Sankey Otto F, Tsen Shaw-Wei D, Lin Nien-Tsung, Kiang Juliann G
Arizona State University, Department of Physics, Tempe, Arizona 85287-1504, USA.
J Biomed Opt. 2007 Mar-Apr;12(2):024009. doi: 10.1117/1.2718935.
Raman spectroscopy is used to study low-wave-number (</=20 cm(-1)) acoustic vibrations of the M13 phage. A well-defined Raman line is observed at around 8.5 cm(-1). The experimental results are compared with theoretical calculations based on an elastic continuum model and appropriate Raman selection rules derived from a bond polarizability model. The observed Raman mode is shown to belong to one of the Raman-active axial modes of the M13 phage protein coat. It is expected that the detection and characterization of this low-frequency vibrational mode can be used for applications in biomedical nanotechnology such as for monitoring the process of virus functionalization and self-assembly.
拉曼光谱法用于研究M13噬菌体的低波数（≤20 cm⁻¹）声学振动。在约8.5 cm⁻¹处观察到一条明确的拉曼谱线。将实验结果与基于弹性连续介质模型的理论计算以及从键极化率模型推导的适当拉曼选择规则进行了比较。观察到的拉曼模式被证明属于M13噬菌体蛋白质外壳的拉曼活性轴向模式之一。预计这种低频振动模式的检测和表征可用于生物医学纳米技术中的应用，例如监测病毒功能化和自组装过程。
