Tsen K T, Dykeman Eric C, Sankey Otto F, Lin Nien-Tsung, Tsen Shaw-Wei D, Kiang Juliann G
Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287-1504, USA.
Virol J. 2006 Sep 22;3:79. doi: 10.1186/1743-422X-3-79.
Recently, a technique which departs radically from conventional approaches has been proposed. This novel technique utilizes biological objects such as viruses as nano-templates for the fabrication of nanostructure elements. For example, rod-shaped viruses such as the M13 phage and tobacco mosaic virus have been successfully used as biological templates for the synthesis of semiconductor and metallic nanowires.
Low wave number (<or= 20 cm-1) acoustic vibrations of the M13 phage have been studied using Raman spectroscopy. The experimental results are compared with theoretical calculations based on an elastic continuum model and appropriate Raman selection rules derived from a bond polarizability model. The observed Raman mode has been shown to belong to one of the Raman-active axial torsion modes of the M13 phage protein coat.
It is expected that the detection and characterization of this low frequency vibrational mode can be used for applications in nanotechnology such as for monitoring the process of virus functionalization and self-assembly. For example, the differences in Raman spectra can be used to monitor the coating of virus with some other materials and nano-assembly process, such as attaching a carbon nanotube or quantum dots.
最近，有人提出了一种与传统方法截然不同的技术。这种新技术利用诸如病毒等生物物体作为制造纳米结构元件的纳米模板。例如，棒状病毒如M13噬菌体和烟草花叶病毒已成功用作合成半导体和金属纳米线的生物模板。
利用拉曼光谱研究了M13噬菌体的低波数（≤20厘米-1）声振动。将实验结果与基于弹性连续介质模型以及从键极化率模型导出的适当拉曼选择规则的理论计算进行了比较。观察到的拉曼模式已被证明属于M13噬菌体蛋白质外壳的拉曼活性轴向扭转模式之一。
预计这种低频振动模式的检测和表征可用于纳米技术应用，如监测病毒功能化和自组装过程。例如，拉曼光谱的差异可用于监测病毒与其他一些材料的包覆以及纳米组装过程，如附着碳纳米管或量子点。
