Agbaje I M, Rogers D A, McVicar C M, McClure N, Atkinson A B, Mallidis C, Lewis S E M
Reproductive Medicine Research Group, Centre for Clinical and Population Sciences, Queen's University of Belfast, Institute of Clinical Science, and Regional Centre for Endocrinology and Diabetes, Royal Victoria Hospital, UK.
Hum Reprod. 2007 Jul;22(7):1871-7. doi: 10.1093/humrep/dem077. Epub 2007 May 3.
Diabetes mellitus (DM) is increasing in men of reproductive age. Despite this, the prevalence of diabetes in men attending fertility clinics is largely unknown. Furthermore, studies examining the effects of DM on sperm fertility potential have been limited to conventional semen analysis.
Conventional semen analysis (semen volume, sperm count, motility and morphology) was performed for 27 diabetic (mean age 34+/-2 years) and 29 non-diabetic subjects (control group, men undergoing routine infertility investigations, mean age 33+/-1 years). Nuclear DNA (nDNA) fragmentation was assessed using the alkaline Comet assay and mitochondrial DNA (mtDNA) deletions by Long-PCR.
Other than a small, but significant, reduction in semen volume in diabetic men (2.6 versus 3.3 ml; P<0.05), conventional semen parameters did not differ significantly from control subjects. Diabetic subjects had significantly higher mean nDNA fragmentation (53 versus 32%; P<0.0001) and median number of mtDNA deletions (4 versus 3; P<0.05) compared with control subjects.
Diabetes is associated with increased sperm nuclear and mtDNA damage that may impair the reproductive capability of these men.
糖尿病（DM）在育龄男性中的发病率正在上升。尽管如此，前往生育诊所就诊的男性糖尿病患病率在很大程度上仍不清楚。此外，研究糖尿病对精子生育潜力影响的研究仅限于传统的精液分析。
对27名糖尿病患者（平均年龄34±2岁）和29名非糖尿病受试者（对照组，接受常规不孕症检查的男性，平均年龄33±1岁）进行传统精液分析（精液量、精子计数、活力和形态）。使用碱性彗星试验评估核DNA（nDNA）片段化，并通过长链聚合酶链反应评估线粒体DNA（mtDNA）缺失。
除糖尿病男性的精液量有小幅但显著的减少外（2.6毫升对3.3毫升；P<0.05），传统精液参数与对照组无显著差异。与对照组相比，糖尿病受试者的平均nDNA片段化显著更高（53%对32%；P<0.0001），mtDNA缺失的中位数也更高（4对3；P<0.05）。
糖尿病与精子核DNA和mtDNA损伤增加有关，这可能会损害这些男性的生殖能力。
