Bonnin P J
C R Seances Soc Biol Fil. 1975;169(3):548-52.
The endocrine gland of the testis of Gobius niger L. was cultured separately for three or six days by dissecting out from the seminal mass. During the sexual resting periode, the explantation don't induce histological changes; however, during the phase of high sexual activity, the explantation involves marked asthenic signs. This phenomenon is in all cases reversed by the association with pituitaries, even after a preliminary isolated culture of three days. The association with pituitaries increases also the functional activity of the explants compared with the controls tested by measuring the total amount of testosterone contained in pooled culture media, or the global metabolism of 14C sodium acetate.
通过从精巢中分离出黑口虾虎鱼（Gobius niger L.）的睾丸内分泌腺，分别培养三天或六天。在性休止期，外植体不会引起组织学变化；然而，在性活动旺盛期，外植体会出现明显的衰弱迹象。即使经过三天的初步单独培养，与垂体联合培养后，这种现象在所有情况下都会逆转。与垂体联合培养相比，通过测量合并培养基中睾酮的总量或14C醋酸钠的整体代谢来测试对照，联合培养还增加了外植体的功能活性。
