Bonnin J P
C R Acad Hebd Seances Acad Sci D. 1977 May 9;284(18):1827-30.
After the interstitial tissue was cultivated, the radio-immunoassay revealed in the media very small quantities of free testosterone but much larger quantities of testosterone conjugates as glucuronids and sulfates. Association of pituitaries increases greatly the secretion. 12-13 days precultivated pituitaires retain close to 70% of their stimulating potency.
间质组织培养后,放射免疫分析显示培养基中游离睾酮含量极少,但睾酮结合物(如葡萄糖醛酸苷和硫酸盐)含量要高得多。垂体联合培养可显著增加分泌量。预培养12 - 13天的垂体仍保留近70%的刺激活性。