Tang Sanyuan, Guan Rongzhan, Zhang Hongsheng, Huang Ji
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing, 210095, P. R. China.
Biotechnol Lett. 2007 Sep;29(9):1417-24. doi: 10.1007/s10529-007-9391-9. Epub 2007 May 4.
Three cDNAs of DsFAD3, DsFAD7 and DsFAD8, encoding omega-3 fatty acid desaturases, which are the key enzymes for the conversion of linoleic to alpha-linolenic acid (18:3n-3), were isolated from Descurainia sophia using RACE-PCR. Tissue-specific expression analysis revealed that DsFAD3 and DsFAD7 genes were expressed in all tissues and at a high level in stems, leaves and young siliques, whereas DsFAD8 was moderately expressed in photosynthetic tissues including stems, leaves and young siliques. All three genes were significantly induced by wounding stress and DsFAD3 and DsFAD7 transcript levels were down-regulated by cold, whereas DsFAD8 was increased upon cold stress.
利用RACE-PCR技术从播娘蒿中分离出了3个编码ω-3脂肪酸去饱和酶的cDNA，即DsFAD3、DsFAD7和DsFAD8，这些酶是将亚油酸转化为α-亚麻酸（18:3n-3）的关键酶。组织特异性表达分析表明，DsFAD3和DsFAD7基因在所有组织中均有表达，在茎、叶和幼嫩角果中表达水平较高，而DsFAD8在包括茎、叶和幼嫩角果在内的光合组织中适度表达。所有这三个基因均受到创伤胁迫的显著诱导，DsFAD3和DsFAD7的转录水平在低温下被下调，而DsFAD8在低温胁迫下则升高。
