Vaught J B, Howard E F, Bresnick E
Cancer Res. 1976 Jan;36(1):272-5.
Damage to and repair of DNA isolated from human neonatal and fetal skin cells were measured by alkaline sucrose gradient analysis. 3-Methylcholanthrene did not induce single-strand breaks in DNA of the cells in culture, whereas the 11,12-oxide of 3-methylcholanthrene was very effective in this regard. The cis-1,2-dihydroxy, trans-11,12-dihydroxy, and cis-11,12-dihydroxy derivatives of 3-methylcholanthrene exerted little effect. The breaks in DNA caused by 3-methylcholanthrene oxide occurred during a 60-min incubation period and were repaired during the following 60 min. Methylmethane sulfonate also induced breaks in the DNA within 60 min.
通过碱性蔗糖梯度分析来测定从人类新生儿和胎儿皮肤细胞中分离出的DNA的损伤及修复情况。3-甲基胆蒽不会在培养的细胞DNA中诱导单链断裂，而3-甲基胆蒽的11,12-氧化物在这方面非常有效。3-甲基胆蒽的顺式-1,2-二羟基、反式-11,12-二羟基和顺式-11,12-二羟基衍生物作用很小。3-甲基胆蒽氧化物引起的DNA断裂发生在60分钟的孵育期内，并在随后的60分钟内得到修复。甲磺酸甲酯也在60分钟内诱导DNA断裂。
