Josephsen Gary D, Josephsen Kelly A, Beilman Greg J, Taylor Jodie H, Muiler Kristine E
Section of Surgical Critical Care, Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA.
Microsc Microanal. 2005 Dec;11(6):500-5. doi: 10.1017/S1431927605050567.
This is a report of the adaptation of microwave processing in the preparation of liver biopsies for transmission electron microscopy (TEM) to examine ultrastructural damage of mitochondria in the setting of metabolic stress. Hemorrhagic shock was induced in pigs via 35% total blood volume bleed and a 90-min period of shock followed by resuscitation. Hepatic biopsies were collected before shock and after resuscitation. Following collection, biopsies were processed for TEM by a rapid method involving microwave irradiation (Giberson, 2001). Samples pre- and postshock of each of two animals were viewed and scored using the mitochondrial ultrastructure scoring system (Crouser et al., 2002), a system used to quantify the severity of ultrastructural damage during shock. Results showed evidence of increased ultrastructural damage in the postshock samples, which scored 4.00 and 3.42, versus their preshock controls, which scored 1.18 and 1.27. The results of this analysis were similar to those obtained in another model of shock (Crouser et al., 2002). However, the amount of time used to process the samples was significantly shortened with methods involving microwave irradiation.
这是一份关于将微波处理应用于制备肝脏活检样本以用于透射电子显微镜（TEM）检查代谢应激状态下线粒体超微结构损伤的报告。通过放血35%总血容量诱导猪发生失血性休克，随后进行90分钟的休克及复苏。在休克前和复苏后采集肝脏活检样本。采集后，通过一种涉及微波辐射的快速方法（Giberson，2001）对活检样本进行TEM处理。使用线粒体超微结构评分系统（Crouser等人，2002）对两只动物休克前后的样本进行观察和评分，该系统用于量化休克期间超微结构损伤的严重程度。结果显示，休克后样本的超微结构损伤增加，评分为4.00和3.42，而休克前对照样本评分为1.18和1.27。该分析结果与另一个休克模型（Crouser等人，2002）中获得的结果相似。然而，采用涉及微波辐射的方法处理样本所花费的时间显著缩短。
