Drost M
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610-0136, USA.
Theriogenology. 2007 Aug;68(3):450-3. doi: 10.1016/j.theriogenology.2007.04.013. Epub 2007 May 3.
Embryo transfer techniques in water buffalo were derived from those in cattle. However, the success rate is much lower in buffaloes, due to their inherent lower fertility and poor superovulatory response. The buffalo ovary has a smaller population of recruitable follicles at any given time than the ovary of the cow (89% fewer at birth). In addition, estrus detection is problematic. Progress in the field of embryo transfer in water buffalo has been slow, and is primarily due to a poor response to superovulation. The average yield of transferable embryos is less than one per superovulated donor. In vitro embryo production could considerably improve the efficacy and logistics of embryo production. The technique of Ovum Pick Up is superior to superovulation; it can yield more transferable embryos per donor on a monthly basis (2.0 versus 0.6). The feasibility of intergeneric embryo transfer between buffalo and cattle has been investigated. No pregnancy resulted after transfer of 13 buffalo embryos to synchronized Holstein heifers. Preliminary successes with nucleus transfer of Bubalus bubalis fetal and adult somatic nuclei into enucleated bovine oocytes and subsequent development to the blastocyst stage have been reported.
水牛的胚胎移植技术源自牛的胚胎移植技术。然而，由于水牛本身繁殖力较低且超数排卵反应不佳，其成功率要低得多。在任何给定时间，水牛卵巢中可募集卵泡的数量都比母牛的卵巢少（出生时少89%）。此外，发情检测也存在问题。水牛胚胎移植领域的进展一直缓慢，主要原因是对超数排卵反应不佳。每个超排供体可移植胚胎的平均产量不到一个。体外胚胎生产可以显著提高胚胎生产的效率和后勤保障。采卵技术优于超数排卵；每月每个供体可产生更多可移植胚胎（分别为2.0个和0.6个）。人们已经研究了水牛和牛之间属间胚胎移植的可行性。将13个水牛胚胎移植到同期发情的荷斯坦小母牛体内后未受孕。有报道称，已成功将水牛胎儿和成体体细胞核移植到去核牛卵母细胞中，并随后发育至囊胚阶段。
