Pakistan Scientific and Technological Information Center, Quaid-i-Azam University Campus, Islamabad, 44000, Pakistan.
Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Center, Islamabad, 45500, Pakistan.
Vet Res Commun. 2021 Sep;45(2-3):47-74. doi: 10.1007/s11259-021-09789-0. Epub 2021 Apr 30.
We explored different aspects of buffalo spermatozoa during cryopreservation. The meta-data comprised of 285 studies, published from January 2008 to March 2020. A free web tool CADIMA as well as PRISMA 2009 Flow Diagram were used for carrying out this study. The inter-reviewer agreement among studies allocated was satisfactory for criteria A (selection bias), B (performance bias), C (detection bias) and D (attrition bias), respectively. India led the percent (%) research ladder with 34.4, followed by Pakistan (29.5), Egypt (12.3), Iran (7.7), Italy (5.6), Indonesia (3.2), China (2.1), Brazil (1.4), Thailand (1.1), Philippines and Bulgaria (0.7 each), Saudi Arabia, Turkey, Vietnam, and USA (0.4 each). Among four categories of studies, Group-1 evaluated only supplements/additives/media in the freezing semen extender (n = 191/285; 67.02%); Group-2 conducted in vivo fertilization (n = 62/285; 21.75%) and Group-3 conducted in vitro fertilization/ cleavage rate/penetration rate/ blastocyst yields (n = 28/285; 9.82%) with their specific cryodiluents/media, respectively. Group-4 conducted different experimental supplements/additives/media and carried out both in vitro and in vivo fertilization simultaneously (n = 4/285; 1.40%). Conventional spermatozoa cryopreservation was reported by 51.9% studies followed by programmable fast freezing by 20.7% studies. A few leading extender types included BioXcell (3.9%); Soyamilk-skim (3.5%); and Andromed (2.1%). The study also describes French straws for semen filling, cooling temperatures, extension time, equilibration time, cryopreservation stages, thawing temperatures, seasons, thawing time, and stains used during semen evaluation assays. The study concludes that the research on spermatozoa cryopreservation of buffalo is largely conducted at quality level and a need of applying these findings for evaluation of fertility potential (in vivo and in vitro) is indispensable for effective genetic improvement.
我们探讨了水牛精子在冷冻保存过程中的不同方面。元数据包括 285 项研究，这些研究发表于 2008 年 1 月至 2020 年 3 月。我们使用了免费的网络工具 CADIMA 以及 PRISMA 2009 流程图来进行这项研究。对研究分配的各项标准（选择偏倚、检测偏倚、实施偏倚和失访偏倚）进行的研究间评估一致性均令人满意。印度以 34.4%的比例领先研究阶梯，其次是巴基斯坦（29.5%）、埃及（12.3%）、伊朗（7.7%）、意大利（5.6%）、印度尼西亚（3.2%）、中国（2.1%）、巴西（1.4%）、泰国（1.1%）、菲律宾和保加利亚（各 0.7%）、沙特阿拉伯、土耳其、越南和美国（各 0.4%）。在这四类研究中，第一组仅评估冷冻精液稀释液中的补充剂/添加剂/培养基（n=191/285；67.02%）；第二组进行体内受精（n=62/285；21.75%），第三组分别进行体外受精/卵裂率/穿透率/囊胚产率（n=28/285；9.82%），并使用各自特定的冷冻液/培养基（n=4/285；1.40%）。常规精子冷冻保存的报道占 51.9%，可编程快速冷冻保存的报道占 20.7%。少数主要的扩展类型包括 BioXcell（3.9%）、大豆奶脱脂（3.5%）和 Andromed（2.1%）。该研究还描述了用于精液填充的法国 straws、冷却温度、扩展时间、平衡时间、冷冻保存阶段、解冻温度、季节、解冻时间以及精液评估试验中使用的染色剂。该研究得出的结论是，水牛精子冷冻保存的研究主要在质量水平上进行，需要将这些发现应用于生育能力（体内和体外）的评估，这对于有效的遗传改良是必不可少的。
