Zhang Qingli, Li Fuhua, Zhang Jiquan, Wang Bing, Gao Hongwei, Huang Bingxin, Jiang Hao, Xiang Jianhai
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, People's Republic of China.
Mol Immunol. 2007 Jul;44(14):3501-9. doi: 10.1016/j.molimm.2007.03.014. Epub 2007 May 3.
Peroxiredoxin (Prx) is known to be an antioxidant protein that protects the organisms against various oxidative stresses and functions in intracellular signal transduction. A Prx gene was firstly isolated in the crustacean, Chinese shrimp Fenneropenaeus chinensis. The full-length cDNA consists of 942bp with a 594bp open reading frame, encoding 198 amino acids. The molecular mass of the deduced amino acid is 22041.17Da with an estimated pI of 5.17. Sequence comparison showed that Prx of F. chinensis shares 76%, 73% and 72% identity with that of Aedes aegypti, Branchiostoma belcheri tsingtaunese and Drosophila melanogaster, respectively. Northern blot analysis revealed the presence of Prx transcripts of F. chinensis in all tissues examined. Real-time PCR analysis indicated that the Prx showed different expression profiles in shrimp hemocytes and hepatopancreas after artificial infection with Vibrio anguillarum. In addition, a fusion protein containing Prx was produced in vitro. LC-ESI-MS analysis showed that four peptide fragments of the recombinant protein were identical to the corresponding sequence of F. chinensis Prx. And the purified recombinant proteins were shown to reduce H(2)O(2) in the presence of dithiothreitol.
过氧化物酶（Prx）是一种抗氧化蛋白，可保护生物体免受各种氧化应激，并在细胞内信号转导中发挥作用。首次在甲壳类动物中国对虾（Fenneropenaeus chinensis）中分离出一个Prx基因。其全长cDNA由942bp组成，开放阅读框为594bp，编码198个氨基酸。推导的氨基酸分子量为22041.17Da，估计pI为5.17。序列比较表明，中国对虾的Prx与埃及伊蚊、青岛文昌鱼和黑腹果蝇的Prx分别具有76%、73%和72%的同一性。Northern印迹分析显示，在所检测的所有组织中均存在中国对虾Prx转录本。实时PCR分析表明，在用鳗弧菌人工感染后，Prx在中国对虾血细胞和肝胰腺中表现出不同的表达谱。此外，体外产生了一种含有Prx的融合蛋白。LC-ESI-MS分析表明，重组蛋白的四个肽段与中国对虾Prx的相应序列相同。并且纯化的重组蛋白在二硫苏糖醇存在下可还原H(2)O(2)。
