Cong Ming, Ni Duojiao, Song Linsheng, Wang Lingling, Zhao Jianmin, Qiu Limei, Li Ling
Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd, Qingdao, 266071, Shandong, China.
Mol Biol Rep. 2009 Jul;36(6):1451-9. doi: 10.1007/s11033-008-9335-z. Epub 2008 Aug 14.
Peroxiredoxin V (PRX V) is known as an atypical 2-cysteine peroxiredoxin that protects the organisms against various oxidative stresses and functions in signal transduction. The cDNA of a PRX V gene (designated as CfPRX) was cloned from scallop Chlamys farreri. The full-length sequence of CfPRX cDNA was of 2,179 bp with a 564 bp open reading frame encoding a peptide of 187 amino acids. Sequence comparison showed that CfPRX shared higher identities with PRX Vs than that with other isoforms of PRX, indicating CfPRX was a member of the PRX V family. Fluorescent real-time quantitative PCR analysis revealed the presence of CfPRX transcripts in gill filaments, adductor muscle, heart, gonad, kidney and hemocytes, and the stimulation of Listonella anguillarum significantly (P < 0.01) enhanced the mRNA expression of CfPRX in hemocyte. These results indicated that CfPRX was a constitutive and inducible acute-phase protein which was involved in the immune resistance to L. anguillarum stimulation.
过氧化物氧还蛋白V（PRX V）是一种非典型的2-半胱氨酸过氧化物氧还蛋白，可保护生物体免受各种氧化应激，并在信号转导中发挥作用。从栉孔扇贝中克隆了PRX V基因（命名为CfPRX）的cDNA。CfPRX cDNA的全长序列为2179 bp，开放阅读框为564 bp，编码一个由187个氨基酸组成的肽段。序列比较表明，CfPRX与PRX V的同源性高于与PRX其他同工型的同源性，表明CfPRX是PRX V家族的成员。荧光实时定量PCR分析显示，CfPRX转录本存在于鳃丝、闭壳肌、心脏、性腺、肾脏和血细胞中，鳗弧菌刺激显著（P < 0.01）增强了血细胞中CfPRX的mRNA表达。这些结果表明，CfPRX是一种组成型和诱导型急性期蛋白，参与了对鳗弧菌刺激的免疫抵抗。
