通过头孢西丁纸片扩散法和PBP2a乳胶凝集试验评估mecA阳性金黄色葡萄球菌对甲氧西林的耐药性，并比较mecA与femA、femB、femX阳性情况。

Evaluation of methicillin resistance by cefoxitin disk diffusion and PBP2a latex agglutination test in mecA-positive Staphylococcus aureus, and comparison of mecA with femA, femB, femX positivities.

作者信息

Akcam Fusun Zeynep, Tinaz Gulgun Bosgelmez, Kaya Onur, Tigli Arzu, Ture Ebru, Hosoglu Salih

机构信息

Department of Infectious Disease and Clinical Microbiology, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey.

出版信息

Microbiol Res. 2009;164(4):400-3. doi: 10.1016/j.micres.2007.02.012. Epub 2007 May 3.

DOI:10.1016/j.micres.2007.02.012

Abstract

Methicillin resistance in staphylococci is primarily due to the presence of a mecA gene which encodes the novel penicillin binding protein2a. Some chromosomal factors such as femA and femB also participate in the expression of methicillin resistance. This study was designed to detect methicillin resistance by cefoxitin disk diffusion and penicillin binding protein2a latex agglutination methods, and to compare mecA, femA, femB and femX gene positivities. A total of 60 MRSA isolates were included in the evaluation. PCR analysis showed that all isolates were positive for mecA and femA genes. Seven of these isolates tested negative by the latex agglutination test. Fifteen isolates were positive for femB and 28 isolates for femX gene. This study implicated that for the determination of methicillin resistance, latex agglutination test is the least reliable method when compared to PCR and cefoxitin disk diffusion test. femA gene shows more correlation than femB and femX with methicillin resistance.

摘要

葡萄球菌对甲氧西林耐药主要是由于存在mecA基因，该基因编码新型青霉素结合蛋白2a。一些染色体因素如femA和femB也参与甲氧西林耐药的表达。本研究旨在通过头孢西丁纸片扩散法和青霉素结合蛋白2a乳胶凝集法检测甲氧西林耐药性，并比较mecA、femA、femB和femX基因的阳性率。共有60株耐甲氧西林金黄色葡萄球菌（MRSA）分离株纳入评估。PCR分析显示所有分离株的mecA和femA基因均为阳性。其中7株分离株乳胶凝集试验检测为阴性。15株分离株femB基因阳性，28株分离株femX基因阳性。本研究表明，对于甲氧西林耐药性的测定，与PCR和头孢西丁纸片扩散试验相比，乳胶凝集试验是最不可靠的方法。femA基因与甲氧西林耐药性的相关性比femB和femX更高。