Ba B B, Ducint D, Fourtillan M, Saux M C
Pharmacokinetic and Clinical Pharmacy Laboratory, Haut Lévêque Hospital and University Victor Segalen Bordeaux II, Pessac, France.
J Chromatogr B Biomed Sci Appl. 1998 Sep 4;714(2):317-24. doi: 10.1016/s0378-4347(98)00192-3.
An isocratic high-performance liquid chromatographic method with column switching and direct injection has been developed to determine ciprofloxacin in plasma and Mueller-Hinton broth. An on-line dilution of the sample was performed with a loading mobile phase consisting of 173 mM phosphoric acid. The analyte was retained on a LiChrocart 4-4 precolumn filled with a LiChrospher 100 RP18, 5 microm. An electric-actuated system with two six-port valves allowed a clean-up step with a mixture 20 mM phosphate buffer (pH 3.5)-methanol (97:3, v/v) and the transfer of the analyte by a back-flush mode to a 150 x 4.6 mm I.D. column packed with a Kromasil C8 5 microm, using a mobile phase of 20 mM phosphate buffer (pH 3.5)-acetonitrile (85:15, v/v). Fluorescence detection allowed a quantification limit of 0.078 microg/ml with a 40-microl sample size. The method was evaluated to determine its usefulness in studying the pharmacokinetic/pharmacodynamic behaviour of ciprofloxacin in an in vitro model.
已开发出一种采用柱切换和直接进样的等度高效液相色谱法，用于测定血浆和穆勒-欣顿肉汤中的环丙沙星。使用由173 mM磷酸组成的上样流动相在线稀释样品。分析物保留在填充有5微米LiChrospher 100 RP18的LiChrocart 4-4预柱上。带有两个六通阀的电动系统允许用20 mM磷酸盐缓冲液（pH 3.5）-甲醇（97:3，v/v）混合物进行净化步骤，并通过反冲模式将分析物转移至内径为150×4.6 mm、填充有5微米Kromasil C8的色谱柱，流动相为20 mM磷酸盐缓冲液（pH 3.5）-乙腈（85:15，v/v）。荧光检测在进样量为40微升时的定量限为0.078微克/毫升。对该方法进行了评估，以确定其在体外模型中研究环丙沙星药代动力学/药效学行为的实用性。
