Issa João Paulo Mardegan, do Nascimento Cássio, Bentley Maria Vitória Lopes Badra, Del Bel Elaine Aparecida, Iyomasa Mamie Mizusaki, Sebald Walter, de Albuquerque Rubens Ferreira
Faculdade de Odontologia, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brasil.
Micron. 2008 Jun;39(4):373-9. doi: 10.1016/j.micron.2007.03.008. Epub 2007 Mar 24.
This study evaluated the quantity and quality of newly formed bone, stimulated by rhBMP-2 in combination with monoolein or chitosan gel as carriers, in critical bone defects created in 36 Wistar rat mandibles. Two weeks after surgery, the animals were anesthetized with 37.5% urethane submitted to perfusion and the hemi-mandibles removed for histological and histomorphometrical analysis. The results showed that there was a statistical difference between groups of animals receiving or not rhBMP-2 (p<0.05). Newly formed bone was more intense in the occlusal region, followed by the basal and middle regions, respectively. Both carriers, monoolein and chitosan gels were adequate for defect filling and control of protein release.
本研究评估了在36只Wistar大鼠下颌骨造成的临界骨缺损中，重组人骨形态发生蛋白-2（rhBMP-2）与单油酸甘油酯或壳聚糖凝胶作为载体联合使用时，新形成骨的数量和质量。术后两周，用37.5%的乌拉坦麻醉动物，进行灌注，然后取出半侧下颌骨进行组织学和组织形态计量学分析。结果显示，接受或未接受rhBMP-2的动物组之间存在统计学差异（p<0.05）。新形成的骨在咬合区域更为密集，其次分别是基底区域和中间区域。单油酸甘油酯和壳聚糖凝胶这两种载体都足以填充缺损并控制蛋白质释放。
