McEwen Charles, Gutteridge Steven
DuPont Corporate Center for Analytical Sciences, Wilmington, Delaware 19880, USA.
J Am Soc Mass Spectrom. 2007 Jul;18(7):1274-8. doi: 10.1016/j.jasms.2007.03.032. Epub 2007 Apr 6.
Fungal cells treated with various inhibitors to the ergosterol pathway were analyzed using an Orbitrap mass spectrometer equipped with an atmospheric solids analysis probe (ASAP). The technique is a rapid means for determining which of the multiple steps in the ergosterol pathway were interrupted by an inhibitor. Furthermore, in an inhibitor concentration study, the ASAP method was able to rapidly provide an estimate of the effectiveness of inhibition. In this method the cells are inserted directly into a hot nitrogen stream, thus eliminating extensive sample workup before analysis. Data indicating the point of pathway interruption are obtained in seconds.
使用配备常压固体分析探头（ASAP）的轨道阱质谱仪，对用各种麦角固醇途径抑制剂处理过的真菌细胞进行分析。该技术是一种快速方法，可确定麦角固醇途径中的多个步骤中哪些被抑制剂阻断。此外，在抑制剂浓度研究中，ASAP方法能够快速提供抑制效果的估计。在该方法中，细胞直接插入热氮气流中，从而省去了分析前大量的样品预处理。表明途径阻断点的数据在数秒内即可获得。
