Yu Ying, Lai Yan, Zheng Xiuli, Wu Jianzhong, Long Zhaoyang, Liang Chunsui
School of Chemistry and Environment, South China Normal University, Guangzhou, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2007 Dec 31;68(5):1356-61. doi: 10.1016/j.saa.2007.02.016. Epub 2007 Mar 1.
Luminescent quantum dots (QDs)-semiconductor nanocrystals are a promising alternative to organic dyes for fluorescence-based applications. We have developed procedures to use CdS to encapsulate CdTe and synthesize a new kind of functionalized CdTe/CdS QDs for the quantitative and selective determination of bovine serum albumin (BSA). Maximum fluorescence intensity was produced at pH 6.83, with excitation and emission wavelengths at 336 and 524 nm, respectively. Under optimal conditions, the straight line equation: DeltaF=6.84+62.29C (10(-6) mol dm(-3)) was found between the relative fluorescence intensity and the concentration of BSA in the range of 0-1.2 x 10(-6) mol dm(-3), and the limit of detection was 5.4 x 10(-8) mol dm(-3). Based on this approach, a novel quantitative method for the determination of BSA is presented in this paper.
发光量子点（QDs）——半导体纳米晶体是用于基于荧光的应用中替代有机染料的一种有前景的选择。我们已经开发出使用硫化镉（CdS）包裹碲化镉（CdTe）并合成一种新型功能化碲化镉/硫化镉量子点的方法，用于定量和选择性测定牛血清白蛋白（BSA）。在pH 6.83时产生最大荧光强度，激发波长和发射波长分别为336纳米和524纳米。在最佳条件下，发现在0至1.2×10⁻⁶摩尔每立方分米范围内，相对荧光强度与牛血清白蛋白浓度之间的直线方程为：ΔF = 6.84 + 62.29C（10⁻⁶摩尔每立方分米），检测限为5.4×10⁻⁸摩尔每立方分米。基于此方法，本文提出了一种测定牛血清白蛋白的新型定量方法。
