Li Li, Zhang Fenfen, Ding Yaping, Wang Yaping, Zhang Lulu
Department of Chemistry, Shanghai University, Shanghai, 200444, PR China.
J Fluoresc. 2009 May;19(3):437-41. doi: 10.1007/s10895-008-0430-2. Epub 2008 Oct 30.
Luminescent quantum dots (QDs)-semiconductor nanocrystals were promising alternative to organic dyes for fluorescence-based applications. In this paper, we developed procedures to use mercaptoacetic acid (MAA) to modify ZnSe nanoparticles and made the nanoparticles to be soluble for the quantitative and selective determination of bovine serum albumin (BSA). Maximum fluorescence intensity was produced at pH 7.0, with excitation and emission wavelengths at 242 and 348 nm, respectively. Under optimal conditions, the straight line equation: Delta(F) = 0.38 + 0.34 C (microg/ml) was found between the relative fluorescence intensity and the concentration of BSA in the range of 9.6-124.8 microg/ml, and the limit of detection was 2 microg/ml.
发光量子点（QDs）——半导体纳米晶体是基于荧光应用中有机染料的有前途的替代品。在本文中，我们开发了使用巯基乙酸（MAA）修饰ZnSe纳米颗粒的方法，并使纳米颗粒可溶，用于定量和选择性测定牛血清白蛋白（BSA）。在pH 7.0时产生最大荧光强度，激发波长和发射波长分别为242和348 nm。在最佳条件下，在9.6 - 124.8 μg/ml范围内，相对荧光强度与BSA浓度之间的直线方程为：Δ(F) = 0.38 + 0.34 C（μg/ml），检测限为2 μg/ml。
