自我形成衔接子PCR：一种简单高效的染色体步移方法。
Self-formed adaptor PCR: a simple and efficient method for chromosome walking.
作者信息
Wang Shiming, He Jian, Cui Zhongli, Li Shunpeng
机构信息
Department of Microbiology, Nanjing Agricultural University, No 1 Weigang, Nanjing, PR China.
出版信息
Appl Environ Microbiol. 2007 Aug;73(15):5048-51. doi: 10.1128/AEM.02973-06. Epub 2007 May 4.
Abstract
We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex genomes.
摘要
我们开发了一种自形成衔接子PCR（称为SEFA PCR），可用于染色体步移。大多数扩增的侧翼序列长度超过2.0 kb，有些长达6.0 kb。SEFA PCR简单高效，在复杂基因组中未知序列的分离方面应具有广泛的应用。
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