Hooft P, van de Voorde H, Van Dijck P
Katholieke Universiteit Leuven, Laboratory of Forensic Medicine, Leuven, Belgium.
Forensic Sci Int. 1991 Sep;50(2):263-8. doi: 10.1016/0379-0738(91)90157-e.
The gram-negative aerobic oral bacterial flora of 100 consecutive corpses was isolated. After the identification and culturing of the isolated organisms, blood grouping was performed by the haemagglutination inhibition technique on dried culture smears, the dried culture medium and a dried ethanol extract of the bacteria. Forty-seven of the samples showed a gram-negative aerobic bacterial growth, giving 58 microorganisms of 14 different species. Positive blood grouping results were found in two of them (Escherichia coli and Serratia marcescens), both of type B. It is concluded that occasional mistyping of blood groups on saliva and oral material may be caused by the oral gram-negative aerobic flora, especially if the specimens are contaminated or putrefying.
分离出100具连续尸体的革兰氏阴性需氧口腔细菌菌群。对分离出的微生物进行鉴定和培养后，采用血凝抑制技术对干燥培养涂片、干燥培养基及细菌的干燥乙醇提取物进行血型鉴定。47个样本显示有革兰氏阴性需氧菌生长，共得到14个不同物种的58种微生物。其中两株（大肠杆菌和粘质沙雷氏菌）血型鉴定结果为阳性，均为B型。得出结论，唾液和口腔材料上偶尔出现的血型误判可能是由口腔革兰氏阴性需氧菌群引起的，尤其是当标本被污染或腐败时。
