Separation of A and E prostaglandins by celite partition chromatography for radioimmunoassay.
作者信息
Meldrum D R, Abraham G E
出版信息
Clin Biochem. 1976 Feb;9(1):42-5. doi: 10.1016/s0009-9120(76)80012-4.
PMID:174837
Abstract
This report describes the use of celite partition chromatography to separate the A and E prostaglandins. The stationary phase consists of a glycine-HCL buffer, pH 3.6-3.7. The mobile phase consists of mixtures of ethyl acetate in toluene. At this critical hydrogen ion concentration of the stationary phase, the two prostaglandin groups are clearly separated on the basis of their difference in polarity, with greater than 95% recoveries and only 5% overlap of the elution patterns. It is anticipated that specific radioimmunoassays can be achieved by combining this system with partially specific antisera.
摘要
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