通过硅藻土分配色谱法分离A和E前列腺素用于放射免疫测定。

Separation of A and E prostaglandins by celite partition chromatography for radioimmunoassay.

作者信息

Meldrum D R, Abraham G E

出版信息

Clin Biochem. 1976 Feb;9(1):42-5. doi: 10.1016/s0009-9120(76)80012-4.

Abstract

This report describes the use of celite partition chromatography to separate the A and E prostaglandins. The stationary phase consists of a glycine-HCL buffer, pH 3.6-3.7. The mobile phase consists of mixtures of ethyl acetate in toluene. At this critical hydrogen ion concentration of the stationary phase, the two prostaglandin groups are clearly separated on the basis of their difference in polarity, with greater than 95% recoveries and only 5% overlap of the elution patterns. It is anticipated that specific radioimmunoassays can be achieved by combining this system with partially specific antisera.

摘要

本报告描述了使用硅藻土分配色谱法分离A和E前列腺素。固定相由pH值为3.6 - 3.7的甘氨酸 - 盐酸缓冲液组成。流动相由乙酸乙酯在甲苯中的混合物组成。在固定相的这一关键氢离子浓度下，基于两种前列腺素极性的差异，它们能被清晰分离，回收率大于95%，洗脱模式的重叠率仅为5%。预计通过将该系统与部分特异性抗血清相结合可实现特异性放射免疫测定。