Antisera to prostaglandins - production and characterization.

Antisera to prostaglandins of the A,B, E and F groups were elicited by immunization with prostaglandin-protein conjugates. Antibodies to protaglandins of the A and B groups cross-react with each other, but discriminate these two groups of prostaglandins from those of the E and F groups. Antibodies to protaglandins of the E groups, elicited with prostaglandin E-bovine serum albumin (PGE-BSA) conjugates in rabbits, cross-react with prostaglandins of the A and B groups. This cross-reaction is diminished by immunization of rats or mice with PGE-BSA, or by immunization of rabbits with PGE conjugated to gamma globulin or thyroglobulin. Antibodies to PGF have a marked group specificity and discriminate between this group of prostaglandins and prostaglandins of the A, B and E groups. All the antibodies produced do not discriminate between individual prostaglandins within the group.