Keeler Shamus P, Fried Bernard, Huffman Jane E
Fish & Wildlife Microbiology Laboratory, Department of Biological Sciences, East Stroudsburg University, 200 Prospect St., East Stroudsburg, PA 18301, USA.
Parasitol Res. 2007 Sep;101(4):1131-3. doi: 10.1007/s00436-007-0557-0. Epub 2007 May 5.
Echinostoma caproni metacercarial cysts often need to be stored for several months to a year to meet laboratory requirements. This study examined the bacteria associated with encysted metacercariae stored at 4 degrees C. Bacterial assemblages from the kidney-pericardial region of uninfected snails and assemblages from the site of metacercarial infection in Biomphalaria glabrata were determined. Four species of Gram-negative rods were isolated and identified from the stored cysts to species or genus as Pseudomonas sp., Alcaligenes sp., Enterobacter cloacae, and Aeromonas hydrophila. Gram-positive rods were also isolated and included small slender rods (alpha hemolytic), small coccobacilli (gamma hemolytic), and small rods that exhibited gamma hemolysis. Accumulation of these bacteria on the surface of encysted metacercariae probably contributed to a decreased cyst viability of E. caproni.
卡氏棘口吸虫后尾蚴囊肿通常需要储存数月至一年才能满足实验室要求。本研究检测了在4℃储存的包囊后尾蚴相关细菌。确定了未感染蜗牛肾心包区域的细菌群落以及光滑双脐螺体内后尾蚴感染部位的细菌群落。从储存的囊肿中分离并鉴定出四种革兰氏阴性杆菌，分别为假单胞菌属、产碱菌属、阴沟肠杆菌和气单胞菌属。还分离出革兰氏阳性杆菌，包括小的细长杆菌（α溶血）、小的球杆菌（γ溶血）和表现出γ溶血的小杆菌。这些细菌在包囊后尾蚴表面的聚集可能导致卡氏棘口吸虫囊肿活力下降。
