Li Shunyu, Kang Hyoun Woo, Choi Min-Ho, Hong Sung-Tae
Department of Parasitology and Tropical Medicine, Yanbian University, 133000, Yanji, Jilin Province, People's Republic of China.
Parasitol Res. 2006 Dec;100(1):25-9. doi: 10.1007/s00436-006-0242-8. Epub 2006 Jun 10.
Clonorchis sinensis metacercariae must often be stored for several months to meet laboratory requirements. In the present study, the authors evaluated morphological changes and infectivities of metacercariae kept in different storage solutions for 1, 3, and 6 months, i.e., PBS (phosphate buffered saline), PBS + antibiotics, PBS + iodoacetic acid (IAA), and PBS + antibiotics + IAA. Metacercariae stored in PBS containing antibiotics showed significantly higher survival rates and infectivities than those stored in PBS alone after 3 or 6 months. The metacercariae that had been stored for 6 months in solutions that were refreshed monthly had also significantly higher survival and infection rates than those stored in nonrefreshed solutions. Moreover, the addition of IAA, an inhibitor of cysteine proteinase, to these storage solutions reduced the survival and infectivity. The results of the present study confirm that the viabilities of C. sinensis metacercariae are best maintained in storage solutions containing antibiotics that are regularly refreshed.
华支睾吸虫囊蚴通常必须储存数月以满足实验室要求。在本研究中，作者评估了囊蚴在不同储存溶液中保存1、3和6个月后的形态变化和感染力，这些溶液分别是PBS（磷酸盐缓冲盐水）、PBS +抗生素、PBS +碘乙酸（IAA）以及PBS +抗生素+IAA。在3个月或6个月后，储存在含抗生素的PBS中的囊蚴存活率和感染力显著高于单独储存在PBS中的囊蚴。每月更换溶液储存6个月的囊蚴，其存活率和感染率也显著高于储存在未更换溶液中的囊蚴。此外，在这些储存溶液中添加半胱氨酸蛋白酶抑制剂IAA会降低存活率和感染力。本研究结果证实，华支睾吸虫囊蚴在定期更换的含抗生素储存溶液中活力能得到最佳维持。
