Guo Huai-Lan, Yang Xue-Feng, Xu Jian, Wang Jun, Yu Dong, Sun Xiu-Fa
Department of Nutrition and Food Hygiene, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2007 Jan;41(1):21-4.
To study the effects of excess iodine intake on neurogranin expression in cerebrum of filial mice and the intervention of selenium.
Sixty BALB/c mice were divided randomly into four groups with different drinking water: control group (tap water, NC), excess iodine group (3000 microg/L I, EL +), supplementing selenium group (200 microg/L Se, Se +) and the excess iodine plus selenium (3000 microg/L + I 200 microg/L Se, EI + Se +) group. The mice were mated at the end of the fourth month. Serum T4 and T3 were determined on postnatal day 14 and 28. The expression level of neurogranin in filial cerebrum was measured by immunohistochemistry and Western blot.
Serum T4 level in EI (68.78 +/- 11.10 nmol/ L) + was lower significantly than that in NC (100.85 +/- 11.47 nmol/ L) and EI + Se + (93.15 +/- 12.10 nmol/ L) on postnatal day 14. Western blot analysis showed that the relative level of neurogranin in EI + (0.621 +/- 0.041) was lower than that in NC (0.841 +/- 0.039) and EI + Se + (0.781 +/- 0.029) on postnatal day 14 (P < 0.05). No significant difference in serum T4 and neurogranin level between four groups on postnatal day 28.
Excess iodine intake might change the expression of neurogranin in filial cerebrum and the selenium supplementation might alleviate it.
研究过量碘摄入对仔鼠大脑神经颗粒素表达的影响及硒的干预作用。
将60只BALB/c小鼠随机分为四组，给予不同的饮用水：对照组（自来水，NC）、过量碘组（3000μg/L碘，EL +）、补硒组（200μg/L硒，Se +）和过量碘加硒组（3000μg/L碘+200μg/L硒，EI + Se +）。在第四个月末将小鼠配对。在出生后第14天和28天测定血清T4和T3。通过免疫组织化学和蛋白质印迹法检测仔鼠大脑中神经颗粒素的表达水平。
出生后第14天，EI +组血清T4水平（68.78±11.10nmol/L）显著低于NC组（100.85±11.47nmol/L）和EI + Se +组（93.15±12.10nmol/L）。蛋白质印迹分析显示，出生后第14天，EI +组神经颗粒素相对水平（0.621±0.041）低于NC组（0.841±0.039）和EI + Se +组（0.781±0.029）（P<0.05）。出生后第28天，四组血清T4和神经颗粒素水平无显著差异。
过量碘摄入可能会改变仔鼠大脑中神经颗粒素的表达，而补充硒可能会减轻这种改变。
