Harton J A, Litaker W, Frelinger J A, Bishop G A
Department of Microbiology, University of Iowa, Iowa City 52242.
Immunogenetics. 1991;34(6):358-65. doi: 10.1007/BF01787485.
The gene encoding the H-2 Ap class II beta chain was isolated from a B10.P genomic library and sequenced. This gene was also used to construct transfectants of the CH12 lymphoma clone CH12.LX, which express the Abp gene product in association with the endogenous A alpha k chain. We present here the first report of the complete nucleotide coding sequence of Abp. The predicted amino acid sequence of Abp reveals only five residues different from Abq, four of which are present in the mature peptide. These four amino acid changes could account for the differential susceptibility of H-2q vs H-2p mice to the development of collagen-induced arthritis (CIA). Antibodies specific for the transfected Abp protein induce CH12.LX cells to secrete immunoglobulin in the presence of antigen. Comparison of the amino acid sequence with other A beta chains that have been tested in signal transduction experiments suggests that amino acid 9 may be important to the signaling ability of class II A molecules.
编码H-2 Ap II类β链的基因从B10.P基因组文库中分离并测序。该基因还用于构建CH12淋巴瘤克隆CH12.LX的转染体，其与内源性Aαk链一起表达Abp基因产物。我们在此展示了Abp完整核苷酸编码序列的首次报告。Abp的预测氨基酸序列显示与Abq仅有五个残基不同，其中四个存在于成熟肽中。这四个氨基酸变化可能解释了H-2q与H-2p小鼠对胶原诱导性关节炎（CIA）发展的不同易感性。针对转染的Abp蛋白的特异性抗体在存在抗原的情况下诱导CH12.LX细胞分泌免疫球蛋白。将氨基酸序列与在信号转导实验中测试过的其他Aβ链进行比较表明，氨基酸9可能对II类A分子的信号传导能力很重要。
