Iwasaki Shintaro, Takeda Atsushi, Motose Hiroyasu, Watanabe Yuichiro
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, Japan.
FEBS Lett. 2007 May 29;581(13):2455-9. doi: 10.1016/j.febslet.2007.04.051. Epub 2007 Apr 30.
Although decapping is an important process in eukaryotic mRNA turnover, little is known about this process in plants. Here, we identified Arabidopsis thaliana decapping proteins AtDCP1 and AtDCP2 and showed that (I) AtDCP2 is an active decapping enzyme, (II) AtDCP1 interacts with itself, (III) AtDCP1 and AtDCP2 are localized to cytoplasmic foci (putative Arabidopsis processing body), and (IV) AtDCP1 and AtDCP2 are essential for post-embryonic development. Our findings provide new insights into the role of decapping-dependent mRNA turnover.
尽管去帽是真核生物mRNA周转过程中的一个重要环节，但在植物中对这一过程却知之甚少。在此，我们鉴定出了拟南芥去帽蛋白AtDCP1和AtDCP2，并表明：（I）AtDCP2是一种活性去帽酶；（II）AtDCP1可与自身相互作用；（III）AtDCP1和AtDCP2定位于细胞质聚集体（推测为拟南芥加工小体）；（IV）AtDCP1和AtDCP2对胚后发育至关重要。我们的研究结果为依赖去帽的mRNA周转作用提供了新的见解。
