Korte G E, Rappa E, Andracchi S
Department of Ophthalmology, Montefiore Medical Center/Albert Einstein College of Medicine, Bronx, NY 10467.
Invest Ophthalmol Vis Sci. 1991 Dec;32(13):3187-97.
Ultrastructural cytochemistry was used to analyze the polarized distribution of alkaline phosphatase (AP) on the plasma membranes of normal and regenerating retinal pigment epithelium (RPE) and the ciliary body epithelium in rabbits. The AP activity was concentrated on the basolateral plasma membrane in normal RPE. In regenerating RPE (after intravenous administration of sodium iodate to damage the RPE), there was a differential expression of AP activity according to the site on the regenerating epithelial sheet. At the edge of the sheet, where cells were undifferentiated and immature (ie, without the polarized distribution of basolateral and apical plasma membrane specializations seen in normal RPE), no plasma membrane AP activity was observed. The AP activity was reexpressed more proximal in the regenerating sheet. It was first evident where contiguous cells formed junctional complexes and cytologic polarization again became apparent. New AP activity was restricted to the basolateral plasma membrane. Eventually, the polarized distribution of AP activity seen in normal RPE was reestablished. In the ciliary body, AP activity was localized to the basolateral plasma membranes of the outer, nonpigmented epithelial cells. It was concluded (1) that cytochemical activity for nonspecific AP is concentrated on the basolateral plasma membrane domain of normal RPE and the normal pattern of AP activity is lost initially and then reexpressed.
运用超微结构细胞化学方法，分析碱性磷酸酶（AP）在正常及再生的兔视网膜色素上皮（RPE）和睫状体上皮细胞膜上的极化分布。正常RPE中，AP活性集中于基底外侧细胞膜。在再生的RPE（静脉注射碘酸钠损伤RPE后），根据再生上皮片上的部位不同，AP活性存在差异表达。在片层边缘，细胞未分化且不成熟（即没有正常RPE中所见的基底外侧和顶端细胞膜特化的极化分布），未观察到细胞膜AP活性。在再生片层中更靠近近端的部位，AP活性重新表达。首先在相邻细胞形成连接复合体且细胞极化再次明显可见的部位表现明显。新的AP活性局限于基底外侧细胞膜。最终，正常RPE中所见的AP活性极化分布得以重建。在睫状体中，AP活性定位于外层非色素上皮细胞的基底外侧细胞膜。得出结论：（1）非特异性AP的细胞化学活性集中于正常RPE的基底外侧细胞膜结构域，AP活性的正常模式最初丧失，随后重新表达。
