Korte G E, Smith J
Department of Ophthalmology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467.
Experientia. 1993 Sep 15;49(9):789-91. doi: 10.1007/BF01923549.
Type II carbonic anhydrase (CAII) in the cytoplasm of the retinal pigment epithelium (RPE) may contribute to the transport of water and solutes across the RPE. The activity of this enzyme in RPE during its response to damage, e.g., during regeneration, is therefore of interest in understanding retinal disease. Immuno-histochemistry was used to compare CAII activity of normal RPE and RPE experimentally induced to regenerate. In normal rabbits, the RPE stained intensely with a peroxidase-linked antibody specific for human CAII. Regenerating RPE stained less intensely. Within the regenerating epithelium, staining appeared more intense in mature cells than in immature ones, suggesting that CAII activity gradually returns during RPE regeneration.
视网膜色素上皮（RPE）细胞质中的II型碳酸酐酶（CAII）可能有助于水和溶质跨RPE的运输。因此，在RPE对损伤作出反应期间，例如在再生过程中，这种酶的活性对于理解视网膜疾病具有重要意义。免疫组织化学被用于比较正常RPE和实验诱导再生的RPE的CAII活性。在正常兔中，RPE用针对人CAII的过氧化物酶连接抗体染色强烈。再生的RPE染色较弱。在再生上皮内，成熟细胞中的染色比未成熟细胞中的染色更强烈，这表明在RPE再生过程中CAII活性逐渐恢复。
