Martinez M Guadalupe, Cordo Sandra M, Candurra Nélida A
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Ciudad Universitaria, Pabellón II, Piso 4, 1428, Buenos Aires, Argentina.
J Gen Virol. 2007 Jun;88(Pt 6):1776-1784. doi: 10.1099/vir.0.82808-0.
Junín virus (JUNV) entry is conducted by receptor-mediated endocytosis. To explore the cellular entry mechanism of JUNV, inhibitory effects of drugs affecting the main endocytic pathways on JUNV entry into Vero cells were analysed. Compounds that impair clathrin-mediated endocytosis were shown to reduce virus internalization without affecting virion binding. In contrast, drugs that alter lipid-raft microdomains, impairing caveola-mediated endocytosis, were not able to block virus entry. To show direct evidence of JUNV entry, transmission electron microscopy was performed; it showed JUNV particles of about 60-100 nm in membrane depressions that had an electron-dense coating. In addition, JUNV particles were found within invaginations of the plasma membrane and vesicles that resembled those of pits and clathrin-coated vesicles. Taken together, these results demonstrate that clathrin-mediated endocytosis is the main JUNV entry pathway into Vero cells and represent an important contribution to the characterization of the arenavirus multiplication cycle.
胡宁病毒（JUNV）通过受体介导的内吞作用进入细胞。为了探究JUNV的细胞进入机制，分析了影响主要内吞途径的药物对JUNV进入Vero细胞的抑制作用。结果表明，损害网格蛋白介导的内吞作用的化合物可减少病毒内化，但不影响病毒粒子的结合。相反，改变脂筏微结构域、损害小窝蛋白介导的内吞作用的药物不能阻断病毒进入。为了提供JUNV进入细胞的直接证据，进行了透射电子显微镜观察；结果显示，在具有电子致密包膜的膜凹陷中有直径约60 - 100 nm的JUNV颗粒。此外，在质膜内陷和类似于小窝及网格蛋白包被小泡的囊泡中发现了JUNV颗粒。综上所述，这些结果表明网格蛋白介导的内吞作用是JUNV进入Vero细胞的主要途径，为沙粒病毒增殖周期的特征描述做出了重要贡献。
