细胞蛋白在胡宁沙粒病毒进入过程中的作用。

Involvement of cellular proteins in Junin arenavirus entry.

作者信息

Martinez M Guadalupe, Forlenza M Belen, Candurra Nélida A

机构信息

Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.

出版信息

Biotechnol J. 2009 Jun;4(6):866-70. doi: 10.1002/biot.200800357.

DOI:10.1002/biot.200800357

PMID:19548229

Abstract

Junin arenavirus (JUNV) entry is dependent on clathrin-mediated pathways and it relies on the integrity of the actin cytoskeleton as well as the dynamics of microtubules. To determine the method of entry used by this human pathogen, we have demonstrated that in Vero cells JUNV is trafficked via the cellular dynamin 2 (dyn2) endocytic pathway and it is dependent on the Eps15 GTPase. In addition, we have shown that the virus travels through Rab5-mediated early and Rab7-mediated late endosomes in its pH-dependent entry. Altogether, this study gives further inside into the endocytic pathway utilized by the arenavirus JUNV.

摘要

胡宁沙粒病毒（JUNV）的进入依赖于网格蛋白介导的途径，并且它依赖于肌动蛋白细胞骨架的完整性以及微管的动力学。为了确定这种人类病原体所使用的进入方法，我们已经证明，在Vero细胞中，JUNV通过细胞动力蛋白2（dyn2）内吞途径进行运输，并且它依赖于Eps15 GTP酶。此外，我们已经表明，该病毒在其pH依赖性进入过程中穿过Rab5介导的早期内体和Rab7介导的晚期内体。总之，这项研究进一步深入了解了沙粒病毒JUNV所利用的内吞途径。