Yabu M, Senda T, Nonaka Y, Matsukawa N, Okamoto M, Fujita H
Department of Anatomy, Osaka University Medical School, Japan.
Histochemistry. 1991;96(5):391-4. doi: 10.1007/BF00315995.
Using in situ hybridization, localization of the gene transcripts of 11 beta-hydroxylase and aldosterone synthase was investigated in order to clarify the sites for the synthesis of corticosterone (glucocorticoid) and aldosterone (mineralocorticoid) in the rat adrenal cortex. The gene transcript of 11 beta-hydroxylase was localized in all the endocrine cells of the entire adrenal cortex, while that of aldosterone synthase was exclusively confined in zona glomerulosa cells. These results represent that every endocrine cell of all the cortical zones synthesizes 11 beta-hydroxylase which converts 11-deoxycorticosterone to corticosterone, and only glomerulosa cells synthesize aldosterone synthase which produces aldosterone from corticosterone. Thus it is clearly shown that zona glomerulosa cells synthesize mineralocorticoid, while zona fasciculata as well as reticularis cells produce glucocorticoid.
为了阐明大鼠肾上腺皮质中皮质酮（糖皮质激素）和醛固酮（盐皮质激素）的合成位点，采用原位杂交技术研究了11β-羟化酶和醛固酮合酶基因转录本的定位。11β-羟化酶的基因转录本定位于整个肾上腺皮质的所有内分泌细胞中，而醛固酮合酶的基因转录本仅局限于球状带细胞。这些结果表明，所有皮质区的每个内分泌细胞都合成将11-脱氧皮质酮转化为皮质酮的11β-羟化酶，只有球状带细胞合成从皮质酮产生醛固酮的醛固酮合酶。因此，清楚地表明球状带细胞合成盐皮质激素，而束状带和网状带细胞产生糖皮质激素。
