Lee Young Ju, Kim Hyun Jung, Park Cheong Kyu, Kim Ki Seuk, Bae Dong Hwa, Kang Min Su, Cho Jae Keun, Kim Ae Ran, Kim Jong Wan, Kim Byoung Han
College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
J Vet Med Sci. 2007 Apr;69(4):399-404. doi: 10.1292/jvms.69.399.
The purpose of this study was to investigate the biological and genetic characterization of persistent Salmonella isolates in an integrated broiler chicken operation, in an attempt to elucidate the source of contamination. From the breeder farm, the hatchery, the broiler farm and the chicken slaughter house of an integrated broiler chicken operation, a total of 6 serotypes were observed. Although S. Heidelberg was not detected in the broiler farm, it was consistently found in the breeder farm, the hatchery and the chicken slaughter house. Also, S. Enteritidis and S. Senftenberg were found in the hatchery and the chicken slaughter house, and the hatchery and the broiler farm, respectively. S. Gallinarum and S. Blockley were found only in the broiler farm, and S. Virchow was only recovered in the chicken slaughter house. Isolated S. Heidelberg, S. Enteritidis and S. Senftenberg strains were divided into 3, 5 and 7 types, respectively, on the basis of all properties. Especially, S. Senftenberg isolates, divided into four types by their antimicrobial resistance patterns, were all obviously the XbaI PFGE pattern. Also, four S. Enteritidis isolates resistant to nalidixic acid showed a difference in phage type and PFGE pattern. Such a different pattern was shown despite Salmonella isolates originating from an integrated broiler operation, suggesting that further epidemiological studies on many integrated chicken companies in Korea are needed.
本研究的目的是调查一体化肉鸡养殖企业中持续性沙门氏菌分离株的生物学和遗传学特征，以试图阐明污染来源。在一个一体化肉鸡养殖企业的种鸡场、孵化场、肉鸡场和鸡肉屠宰场，共观察到6种血清型。虽然在肉鸡场未检测到海德堡沙门氏菌，但在种鸡场、孵化场和鸡肉屠宰场均持续检测到该菌。此外，肠炎沙门氏菌和森夫滕贝格沙门氏菌分别在孵化场和鸡肉屠宰场、孵化场和肉鸡场被发现。鸡白痢沙门氏菌和布氏沙门氏菌仅在肉鸡场被发现，维尔肖沙门氏菌仅在鸡肉屠宰场被分离到。根据所有特性，分离出的海德堡沙门氏菌、肠炎沙门氏菌和森夫滕贝格沙门氏菌菌株分别分为3型、5型和7型。特别是，森夫滕贝格沙门氏菌分离株根据其抗菌药物耐药模式分为4型，均明显为XbaI PFGE模式。此外，4株对萘啶酸耐药的肠炎沙门氏菌分离株在噬菌体分型和PFGE模式上存在差异。尽管沙门氏菌分离株源自一个一体化肉鸡养殖企业，但仍呈现出这种不同的模式，这表明需要对韩国许多一体化养鸡企业进行进一步的流行病学研究。
