National Veterinary Research and Quarantine Service, Anyang, Gyeonggi, 430-824, Korea.
Poult Sci. 2010 Oct;89(10):2116-22. doi: 10.3382/ps.2010-00918.
Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) has been associated with morbidity and mortality in broiler chickens worldwide. The present study described purulent arthritis of broilers infected with Salmonella Enteritidis and investigated antibiograms and genetic characteristics of Salmonella Enteritidis isolates from epidemiologically related properties such as a hatchery and breeder farm in an attempt to elucidate the source of contamination. Clinical disease and mortality were observed in the affected broiler flock. Mortality was 5.8% until 12 d of age. The birds typically showed lameness with moderately swollen hock joints and footpads. The most prevalent lesions were severely purulent arthritis with polyserositis. Histopathology revealed moderate to severe inflammation in the synovial membrane of leg joints and visceral organs. When the antimicrobial susceptibility test was performed against 7 isolates of Salmonella Enteritidis from broilers, and relevant hatchery and breeder farms by the disk diffusion method using 18 antimicrobial agents, isolates from broiler and breeder farms had the same antibiogram characterized by multiple drug resistance to ampicillin, ceftiofur, cephalothin, gentamycin, nalidixic acid, streptomycin, sulfisoxazole, and tetracycline, whereas isolates from the hatchery were differently resistant to only nalidixic acid. Through the genetic analysis with pulsed-field gel electrophoresis using the restriction enzyme XbaI, Salmonella Enteritidis isolates from both broiler and breeder farms also showed the same PFGE pattern compared with the hatchery isolates resistant to nalidixic acid. As a result, the same PFGE profiles and antibiogram patterns among isolates from broilers and breeder farms provided direct evidence of vertical Salmonella Enteritidis transmission from the contaminated breeder farm to commercial broiler.
肠炎沙门氏菌血清型肠炎（肠炎沙门氏菌）已与世界范围内肉鸡的发病率和死亡率相关。本研究描述了感染肠炎沙门氏菌的肉鸡化脓性关节炎，并调查了来自流行病学相关场所（如孵化场和种鸡场）的肠炎沙门氏菌分离株的药敏谱和遗传特征，试图阐明污染的来源。受感染的肉鸡群中出现了临床疾病和死亡率。死亡率在 12 日龄前为 5.8%。这些鸡通常表现出跛行，跗关节和脚垫中度肿胀。最常见的病变是严重化脓性关节炎伴多发性浆膜炎。组织病理学显示腿部关节和内脏器官的滑膜有中度至重度炎症。当使用 18 种抗菌药物通过圆盘扩散法对来自肉鸡和种鸡场的 7 株肠炎沙门氏菌进行药敏试验时，来自肉鸡和种鸡场的分离株具有相同的药敏谱，对氨苄西林、头孢噻呋、头孢噻吩、庆大霉素、萘啶酸、链霉素、磺胺二甲氧嘧啶和四环素均具有多重耐药性，而来自孵化场的分离株仅对萘啶酸具有不同的耐药性。通过使用 XbaI 限制酶进行脉冲场凝胶电泳的遗传分析，来自肉鸡和种鸡场的肠炎沙门氏菌分离株与仅对萘啶酸耐药的孵化场分离株也显示出相同的 PFGE 模式。结果，来自肉鸡和种鸡场的分离株之间相同的 PFGE 图谱和药敏谱模式为从受污染的种鸡场垂直传播肠炎沙门氏菌到商业肉鸡提供了直接证据。
