Yoshihara Kazuhiro, Morris Agaba, Iraqi Fuad, Naessens Jan
Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan.
J Vet Med Sci. 2007 Apr;69(4):421-3. doi: 10.1292/jvms.69.421.
It is known that different breeds of cattle display differential susceptibilities to Trypanosome congolense infections, and that N'Dama cattle remain more productive after infection than Boran cattle which are more susceptible to T. congolense. Macrophages from both breeds were cultured in vitro and the expressions of a number of cytokines and iNOS mRNA were analyzed using real time RT-PCR after stimulation with antibody-opsonized trypanosomes. No significant difference was seen between the responses of the two breeds. However, RNA levels of TNF-alpha in the IFN-gamma-primed macrophages were about 100-fold higher than those in the non-primed macrophages. A significant ten-fold decrease was seen for the anti-inflammatory cytokine IL-10. These results indicate that priming of the cells with IFN-gamma cause a serious shift toward an inflammatory response.
已知不同品种的牛对刚果锥虫感染表现出不同的易感性，并且恩达马牛在感染后比更易感染刚果锥虫的博拉牛保持更高的生产力。将两个品种的巨噬细胞进行体外培养，在用抗体调理过的锥虫刺激后，使用实时逆转录聚合酶链反应分析多种细胞因子和诱导型一氧化氮合酶mRNA的表达。两个品种的反应之间未观察到显著差异。然而，在经γ干扰素预刺激的巨噬细胞中，肿瘤坏死因子-α的RNA水平比未预刺激的巨噬细胞高约100倍。抗炎细胞因子白细胞介素-10显著下降了10倍。这些结果表明，用γ干扰素对细胞进行预刺激会导致严重转向炎症反应。
