Ohsawa Ikuroh, Ishikawa Masahiro, Takahashi Kumiko, Watanabe Megumi, Nishimaki Kiyomi, Yamagata Kumi, Katsura Ken-Ichiro, Katayama Yasuo, Asoh Sadamitsu, Ohta Shigeo
Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Graduate School of Medicine, Nippon Medical School, 1-396 Kosugi-cho, Nakahara-ku, Kawasaki City 211-8533, Japan.
Nat Med. 2007 Jun;13(6):688-94. doi: 10.1038/nm1577. Epub 2007 May 7.
Acute oxidative stress induced by ischemia-reperfusion or inflammation causes serious damage to tissues, and persistent oxidative stress is accepted as one of the causes of many common diseases including cancer. We show here that hydrogen (H(2)) has potential as an antioxidant in preventive and therapeutic applications. We induced acute oxidative stress in cultured cells by three independent methods. H(2) selectively reduced the hydroxyl radical, the most cytotoxic of reactive oxygen species (ROS), and effectively protected cells; however, H(2) did not react with other ROS, which possess physiological roles. We used an acute rat model in which oxidative stress damage was induced in the brain by focal ischemia and reperfusion. The inhalation of H(2) gas markedly suppressed brain injury by buffering the effects of oxidative stress. Thus H(2) can be used as an effective antioxidant therapy; owing to its ability to rapidly diffuse across membranes, it can reach and react with cytotoxic ROS and thus protect against oxidative damage.
由缺血再灌注或炎症诱导的急性氧化应激会对组织造成严重损伤，而持续性氧化应激被认为是包括癌症在内的许多常见疾病的病因之一。我们在此表明，氢气（H₂）在预防和治疗应用中具有作为抗氧化剂的潜力。我们通过三种独立方法在培养细胞中诱导急性氧化应激。氢气选择性地还原了羟自由基，这是活性氧（ROS）中细胞毒性最强的，并且有效地保护了细胞；然而，氢气不与其他具有生理作用的活性氧发生反应。我们使用了一个急性大鼠模型，其中通过局灶性缺血和再灌注在大脑中诱导氧化应激损伤。吸入氢气气体通过缓冲氧化应激的影响显著抑制了脑损伤。因此，氢气可作为一种有效的抗氧化疗法；由于其能够迅速跨膜扩散，它可以到达细胞毒性活性氧并与之反应，从而防止氧化损伤。
