Kosaka Y, Mimori K, Fukagawa T, Ishikawa K, Etoh T, Katai H, Sano T, Watanabe M, Sasako M, Mori M
Department of Surgery, Medical Institute of Bioregulation, Kyushu University, 4546, Tsurumihara, Beppu 874-0838, Japan.
Br J Cancer. 2007 Jun 4;96(11):1723-8. doi: 10.1038/sj.bjc.6603785. Epub 2007 May 8.
Identification of an isolated tumour cell with metastatic ability is important for predicting the recurrence and prognosis of gastric cancer. A biological marker for evaluating the metastatic ability of gastric cancer cells has not yet been identified. We assessed vascular endothelial growth factor receptor-1 mRNA expression by quantitative real-time reverse transcriptase-polymerase chain reaction. Vascular endothelial growth factor receptor-1 mRNA in peripheral blood was more highly expressed in perioperative metastasis-positive and postoperative recurrence cases than in normal control cases, early cancer cases and nonmetastatic advanced cancer cases. The peripheral blood vascular endothelial growth factor receptor-1 mRNA-positive group was associated with advanced clinical stage, deep invasion beyond the muscularis propria, lymphatic involvement, vascular involvement, lymph node metastasis, positive peritoneal lavage cytology, preoperative metastasis and postoperative recurrence. Flow cytometry analysis disclosed that vascular endothelial growth factor receptor-1 expressing cells in the peripheral blood were more abundant in cancer cases with metastases than in cases without metastases. Our data suggest that the amount of positive cells may provide information on the clinical features of gastric cancer, especially in regard to gastric cancer metastasis.
鉴定具有转移能力的孤立肿瘤细胞对于预测胃癌的复发和预后很重要。目前尚未确定评估胃癌细胞转移能力的生物学标志物。我们通过定量实时逆转录聚合酶链反应评估血管内皮生长因子受体-1 mRNA的表达。围手术期转移阳性和术后复发病例外周血中的血管内皮生长因子受体-1 mRNA表达高于正常对照病例、早期癌症病例和非转移性进展期癌症病例。外周血血管内皮生长因子受体-1 mRNA阳性组与临床晚期、肌层固有层以外的深度浸润、淋巴管受累、血管受累、淋巴结转移、腹腔灌洗细胞学阳性、术前转移和术后复发相关。流式细胞术分析显示，有转移的癌症病例外周血中表达血管内皮生长因子受体-1的细胞比无转移的病例更多。我们的数据表明，阳性细胞的数量可能提供有关胃癌临床特征的信息，尤其是在胃癌转移方面。
