Kahraman Serdar, Düz Bülent, Kayali Hakan, Korkmaz Ahmet, Oter Sükrü, Aydin Ahmet, Sayal Ahmet
Gülhane Askeri Tip Akademisi, Beyin ve Sinir Cerrahisi Anabilim Dali, 06018 Ankara, Turkey.
Neurochem Res. 2007 Sep;32(9):1547-51. doi: 10.1007/s11064-007-9354-5. Epub 2007 May 8.
The effects of hyperbaric oxygen (HBO) therapy or methylprednisolone on the oxidative status were evaluated in experimental spinal cord injury. Clip compression method was used to produce acute spinal cord injury rats. Hyperbaric oxygen was administered twice daily for a total of eight 90 min-sessions at 2.8 atmospheres. Methylprednisolone was first injected with a bolus of 30 mg/kg followed with an infusion rate of 5.4 mg/kg/h for 24 h. Five days after clip application animals were sacrificed and their traumatized spinal cord segment were excised. Tissue levels of thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were evaluated to reflect oxidant/antioxidant status. Non-treated clip-operated animals reflected significantly higher SOD, GSH-Px and TBARS levels that were found to be significantly higher than the sham-operated. Methylprednisolone was not able to lower these levels. HBO administration diminished all measured parameters significantly; however, their levels appeared already to be high when compared with sham animals. According to these results obtained on the 5th day after induction, HBO, but not methylprednisolone, seems to procure prevention against oxidative spinal cord injury.
在实验性脊髓损伤中评估了高压氧（HBO）疗法或甲基强的松龙对氧化状态的影响。采用夹闭压迫法制备急性脊髓损伤大鼠。高压氧每天给药两次，共进行8次90分钟的疗程，压力为2.8个大气压。甲基强的松龙先给予30mg/kg的负荷剂量，然后以5.4mg/kg/h的输注速率持续输注24小时。夹闭后5天处死动物，切除其受伤的脊髓节段。评估硫代巴比妥酸反应性物质（TBARS）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的组织水平以反映氧化/抗氧化状态。未治疗的夹闭手术动物的SOD、GSH-Px和TBARS水平显著高于假手术组，且明显高于假手术组。甲基强的松龙未能降低这些水平。给予HBO可显著降低所有测量参数；然而，与假手术动物相比，其水平似乎已经很高。根据诱导后第5天获得的这些结果，HBO似乎能预防脊髓氧化损伤，而甲基强的松龙则不能。
