Wang Xue-Jian, Chen Jian, Lv Zheng-Bing, Nie Zuo-Ming, Wang Dan, Shen Hong-Dan, Wang Xue-Dong, Wu Xiang-Fu, Zhang Yao-Zhou
Institute of Biochemistry, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China.
J Cell Biochem. 2007 Nov 1;102(4):970-9. doi: 10.1002/jcb.21333.
Cellular retinoic acid binding protein (CRABP) is a member of intracellular lipid-binding protein (iLBP), and closely associated with retinoic acid (RA) activity. We have cloned the CRABP gene from silkworm pupae and studied the interaction between Bombyx mori CRABP (BmCRABP) and all-trans retinoic acid (atRA). The MTT assay data indicated that when BmCRABP is overexpressed in Bm5 cells, the cells dramatically resisted to atRA-induced growth inhibition. Conversely, the cells were sensitive to atRA treatment upon knocking down the BmCRABP expression. Subcellular localization revealed that BmCRABP is a cytoplasm protein, even when treated with atRA, the CRABP still remained in the cytoplasm. These data demonstrated that the function of BmCRABP have an effect on the physiological function of atRA.
细胞视黄酸结合蛋白（CRABP）是细胞内脂质结合蛋白（iLBP）的成员之一，与视黄酸（RA）活性密切相关。我们从家蚕蛹中克隆了CRABP基因，并研究了家蚕CRABP（BmCRABP）与全反式视黄酸（atRA）之间的相互作用。MTT法检测数据表明，当BmCRABP在Bm5细胞中过表达时，细胞对atRA诱导的生长抑制具有显著抗性。相反，敲低BmCRABP表达后，细胞对atRA处理敏感。亚细胞定位显示BmCRABP是一种细胞质蛋白，即使在用atRA处理后，CRABP仍保留在细胞质中。这些数据表明BmCRABP的功能对atRA的生理功能有影响。
