Institute of Life Sciences, Jiangsu University, 301 Xuefu Rd, Zhenjiang, 212013, China,
Cytotechnology. 2014 Jan;66(1):181-91. doi: 10.1007/s10616-013-9551-9. Epub 2013 Mar 26.
The survivin (svv) gene is a newly discovered member of the inhibitors of apoptosis gene family. In recent years, svv has been confirmed to have an anti-apoptosis function and to play a critical role in cell division. We identified a survivin-like gene in the silkworm, Bombyx mori (Bm-svv). In this study, to gain insight into its function, a baculovirus expression system was used to express the Bm-svv gene in insect cell lines. The recombinant viruses were then used as a vector to transform insect cells, and cell activity was determined using the Cell Counting Kit-8 (CCK-8), which is usually employed for detecting mammalian cell number. The results indicated that the Bm-svv gene plays a role in the cell growth arrest or apoptosis induced by viruses. Furthermore, the CCK-8 kit is effective in determining the activity of insect cells.
Survivin（svv）基因是凋亡抑制基因家族中最新发现的成员。近年来，svv 被证实具有抗凋亡功能，并在细胞分裂中起关键作用。我们在桑蚕（Bombyx mori）中鉴定出一个 survivin 样基因（Bm-svv）。在这项研究中，为了深入了解其功能，我们使用杆状病毒表达系统在昆虫细胞系中表达 Bm-svv 基因。然后，将重组病毒用作载体转化昆虫细胞，并使用细胞计数试剂盒（CCK-8）测定细胞活性，CCK-8 试剂盒通常用于检测哺乳动物细胞数量。结果表明，Bm-svv 基因在病毒诱导的细胞生长停滞或凋亡中起作用。此外，CCK-8 试剂盒可有效测定昆虫细胞的活性。
