Schmiedberg S K, Jones L C, Chang D H, Hungerford D S, Frondoza C G
Johns Hopkins Orthopaedics at the Good Samaritan Hospital Suite 201 GSH POB, 5601 Loch Raven Blvd., Baltimore, MD 21239, USA.
Biomed Sci Instrum. 2007;43:104-9.
Wear debris generated from total joint arthroplasty may elicit a granulomatous and inflammatory response and has also been implicated in the development of osteolysis. Technical difficulty in retrieval and isolation of wear material from tissues has hindered the study of their physicochemical properties. The purpose of this study was to retrieve and analyze metallic wear debris from periprosthetic tissue obtained during revision arthroplasty. Tissue from six osteoarthritic patients was obtained during revision arthroplasty. The tissue was minced and then heated in a sodium dodecyl sulfate solution. Undigested tissue was incubated sequentially with papain and pepsin solutions. Metallic wear debris retrieved from the digestion procedure was analyzed by scanning electron microscopy. Wear fragments were seen as irregularly shaped flakes, splinters and polyhedral structures ranging from 1 to 100 microns in size. These structures appeared to be free from non-metallic surface-adherent material. Energy dispersion spectroscopy verified the presence of cobalt, chrome and molybdenum which comprised the implant alloy. Fatigue lines were observed on the surface suggesting brittle wear. Our technique for isolating metallic fragments facilitates the retrieval and preparation of wear debris for analysis of physicochemical properties and how wear debris interacts with cellular elements in surrounding tissue.
全关节置换产生的磨损碎屑可能引发肉芽肿性和炎症反应，并且也被认为与骨溶解的发展有关。从组织中获取和分离磨损材料的技术难题阻碍了对其物理化学性质的研究。本研究的目的是从翻修关节置换术中获得的假体周围组织中获取并分析金属磨损碎屑。在翻修关节置换术中从六名骨关节炎患者身上获取组织。将组织切碎，然后在十二烷基硫酸钠溶液中加热。未消化的组织依次与木瓜蛋白酶和胃蛋白酶溶液孵育。通过扫描电子显微镜分析从消化过程中获取的金属磨损碎屑。磨损碎片呈不规则形状的薄片、碎片和多面体结构，尺寸从1到100微米不等。这些结构似乎没有非金属表面附着材料。能量色散光谱证实了构成植入合金的钴、铬和钼的存在。在表面观察到疲劳线，表明存在脆性磨损。我们分离金属碎片的技术有助于获取和制备磨损碎屑，以分析其物理化学性质以及磨损碎屑与周围组织中的细胞成分如何相互作用。
