Woodall James, Tucci Michelle, Mishra Allan, Benghuzzi Hamed
Department of Orthopedic Surgery and Rehabilitation, University of Mississippi Medical Center, 2500 N. State St. Jackson, MS 39216, USA.
Biomed Sci Instrum. 2007;43:266-71.
Healing injured tissue in the body is a complex process which consists of four distinct phases: hemostasis, inflammation, proliferation, and remodeling. Each of these phases is coordinated by growth factor release and cell to cell interactions. Platelet rich plasma (PRP) is a fraction of plasma that has been isolated and used to enhance regeneration in bone and soft tissues. The healing potential of PRP has been attributed to the release of multiple growth factors from the highly concentrated platelets. While there is strong evidence of the pro-stimulatory effect on the cellular proliferation phase of healing, there is little evidence of the effects of PRP on the inflammatory phase of healing. In this study we investigate the effect that PRP has on macrophage cells in culture and the implications this has on the healing process. We investigate specifically the effects of the separate cellular components of PRP, as a whole and individually, on cell proliferation in human macrophage cells in culture. In contrast to the pro-stimulatory effect that PRP has on cells such as osteoblasts, fibroblasts, and tendon cells; our results show a suppression ofmacrophages by PRP as early as 24 hours after treatment. This suppression was statistically significant (p=0.002) and continued to be significant for the duration of the study. The cell viability results of PRP compared to platelet poor plasma (PPP) and individual components of PRP showed that PRP resulted in a steady increase in viability following the initial insult to the macrophage cells, while the viability of other treatment groups seemed to plateau.
人体受伤组织的愈合是一个复杂的过程，包括四个不同阶段：止血、炎症、增殖和重塑。这些阶段中的每一个都由生长因子释放和细胞间相互作用协调。富血小板血浆（PRP）是血浆的一部分，已被分离出来用于促进骨骼和软组织的再生。PRP的愈合潜力归因于高浓度血小板释放的多种生长因子。虽然有强有力的证据表明其对愈合的细胞增殖阶段有促刺激作用，但几乎没有证据表明PRP对愈合的炎症阶段有影响。在本研究中，我们调查了PRP对培养中的巨噬细胞的影响以及这对愈合过程的影响。我们具体研究了PRP的各个细胞成分作为一个整体以及单独对培养中的人巨噬细胞增殖的影响。与PRP对成骨细胞、成纤维细胞和肌腱细胞等细胞的促刺激作用相反；我们的结果显示，早在治疗后24小时，PRP就对巨噬细胞有抑制作用。这种抑制在统计学上具有显著性（p=0.002），并且在研究期间一直保持显著。与贫血小板血浆（PPP）及PRP的各个成分相比，PRP的细胞活力结果表明，在巨噬细胞最初受到损伤后，PRP导致其活力稳步增加，而其他治疗组的活力似乎趋于平稳。
