经鼻给予浓缩生长因子对诱导嗅觉功能障碍大鼠模型嗅上皮再生的影响。
Effect of intranasal administration of concentrated growth factors on regeneration of the olfactory epithelium in an olfactory dysfunction-induced rat model.
机构信息
Department of Otolaryngology, Head and Neck Surgery, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime, Japan.
Department of Otolaryngology, Ehime Prefectural Niihama Hospital, Niihama, Japan.
出版信息
PLoS One. 2024 Feb 28;19(2):e0298640. doi: 10.1371/journal.pone.0298640. eCollection 2024.
OBJECTIVE
The development of treatments that promote the regenerative capacity of the olfactory epithelium (OE) is desirable. This study aimed to evaluate the effects of intranasal administration of concentrated growth factors (CGFs) in a rat model of olfactory dysfunction.
STUDY DESIGN
Animal study.
METHODS
Nineteen male rats were used. Fourteen olfactory dysfunction models were created by intraperitoneal administration of 3-methylindole. We randomly divided the rats from the olfactory dysfunction model after 1 week into the CGF or saline group; CGFs were administered to seven animals and saline to seven animals. Behavioral assessments using the avoidance test were conducted until day 28 after CGF/saline administration. On day 28, histological evaluation was conducted to determine olfactory epithelial thickness and the olfactory marker protein (OMP)-positive cell count. Five animals were intraperitoneally injected with saline as the control group.
RESULTS
The avoidance rate remained decreased until 28 days after CGF/saline administration, and there was no significant difference between the two groups. Olfactory epithelial thicknesses on day 28 were 38.64 ± 3.17 μm and 32.84 ± 4.50 μm in the CGF and saline groups, respectively. OE thickness was significantly thicker in the CGF group than in the saline group (P = 0.013). The numbers of OMP-positive cells were 40.29 ± 9.77/1.0 × 104 μm2 and 31.00 ± 3.69/1.0 × 104 μm2 in the CGF and saline groups, respectively. The number of OMP+ cells in the CGF group was significantly increased compared with that in the saline group (P = 0.009). Both groups showed no improvement compared with the control group (OE thickness: 54.08 ± 3.36 μm; OMP+ cell count: 56.90 ± 9.91/1.0 × 104 μm2).
CONCLUSIONS
The CGF group showed improved olfactory epithelial thickness and OMP-positive cell numbers compared with that in the saline group.
目的
开发能够促进嗅上皮（OE）再生能力的治疗方法是可取的。本研究旨在评估经鼻给予浓缩生长因子（CGF）在大鼠嗅觉功能障碍模型中的作用。
研究设计
动物研究。
方法
使用 19 只雄性大鼠。通过腹腔内给予 3-甲基吲哚建立 14 个嗅觉功能障碍模型。我们将 1 周后嗅觉功能障碍模型中的大鼠随机分为 CGF 组或生理盐水组；7 只动物给予 CGF，7 只动物给予生理盐水。在给予 CGF/生理盐水后第 28 天进行使用回避测试的行为评估。第 28 天，进行组织学评估以确定嗅上皮厚度和嗅觉标记蛋白（OMP）阳性细胞计数。5 只动物腹腔内注射生理盐水作为对照组。
结果
回避率在给予 CGF/生理盐水后 28 天内持续下降，两组之间无显著差异。第 28 天，CGF 组和生理盐水组的嗅上皮厚度分别为 38.64±3.17μm和 32.84±4.50μm。CGF 组 OE 厚度明显厚于生理盐水组（P=0.013）。OMP 阳性细胞数分别为 40.29±9.77/1.0×104μm2和 31.00±3.69/1.0×104μm2。CGF 组 OMP+细胞数明显高于生理盐水组（P=0.009）。与对照组相比，两组均无改善（OE 厚度：54.08±3.36μm；OMP+细胞计数：56.90±9.91/1.0×104μm2）。
结论
CGF 组与生理盐水组相比，嗅上皮厚度和 OMP 阳性细胞数均有改善。
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