Xu Huiqin, Shen Jian, Liu Hong, Shi Yan, Li Lihua, Wei Min
Department of Pharmacology, College of Pharmacy, Nanjing University of Traditional Chinese Medicine, 282 HanZhong Road, Nanjing 210029, China.
Can J Physiol Pharmacol. 2006 Dec;84(12):1267-73. doi: 10.1139/y06-075.
Advanced glycation end products (AGE) are involved in the alterations of renal mesangial cell (MCs) growth, a feature of early stages of diabetic nephropathy (DN). We postulate that morroniside and loganin, 2 components extracted from Cornus officinalis, may ameliorate the detrimental effects of AGE-induced MCs proliferation by preventing oxidative stress. Rat MCs cultured in AGE milieu were treated with morroniside and loganin. Results showed that morroniside and loganin inhibited AGE-induced MC proliferation as measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Fluorescence microscopy revealed that the morroniside and loganin improved the morphological changes of MCs. Flow cytometric analysis showed that morroniside and loganin inhibited the cell cycle of rat MCs. Furthermore, the level of reactive oxygen species was significantly reduced, and the activities of superoxide dismutase and glutathione peroxidase were markedly increased, whereas the level of malondialdehyde was not significantly reduced. These results suggest that morroniside and loganin regulate MC growth by preventing oxidative stress. Thus, this study provides a molecular basis for the use of morroniside and loganin in the early stages of DN.
晚期糖基化终末产物（AGE）参与了肾系膜细胞（MCs）生长的改变，这是糖尿病肾病（DN）早期阶段的一个特征。我们推测，从山茱萸中提取的两种成分莫诺苷和马钱苷，可能通过预防氧化应激来改善AGE诱导的MCs增殖的有害影响。用莫诺苷和马钱苷处理在AGE环境中培养的大鼠MCs。结果表明，通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）法测定，莫诺苷和马钱苷抑制了AGE诱导的MC增殖。荧光显微镜检查显示，莫诺苷和马钱苷改善了MCs的形态变化。流式细胞术分析表明，莫诺苷和马钱苷抑制了大鼠MCs的细胞周期。此外，活性氧水平显著降低，超氧化物歧化酶和谷胱甘肽过氧化物酶的活性显著增加，而丙二醛水平没有显著降低。这些结果表明，莫诺苷和马钱苷通过预防氧化应激来调节MC生长。因此，本研究为莫诺苷和马钱苷在DN早期的应用提供了分子基础。
